Upstream, Downstream Process Associate, Process Development, Vectors
Company: Yoh, A Day & Zimmermann Company
Location: Medford
Posted on: January 15, 2021
|
|
Job Description:
Process Development Scientist (Upstream/ Downstream, Bioprocess,
Viral Vectors, AAV) 305174
RESPONSIBILITIES:
Bioprocess; Upstream/ Downstream Process Development, process
optimization for manufacturing viral vectors
Vector Engineering/ Viral Vectors. Support vector core Vector
Engineering processes, Process Development
Support vector core vector engineering processes, process
development, and process optimization
Process Development, process optimization, Bioprocess
Upstream/ Downstream Process Development, process optimization for
manufacturing viral vectors
Bioprocess development of AAV vectors focusing, upstream
cultivation and harvest conditions to optimize yield
Working with AAV viral particles, fermentation, plasmid recovery,
purification and formulation, sterilization, and harvesting process
steps
Apply research/ industry knowledge of Lentivirus (LV) and or Adeno
Associated Virus (AAV)
Cell Line maintenance, Cell Line development, Media development; as
cell are transfected with plasmids to generate viral vectors, which
are efficiently harvested
Creating and maintaining stable cell lines
Troubleshooting and optimizing stepwise.
Plasmid Preparation, Buffer, Media Preparation, Aseptic Processing,
growth & maintenance of cell lines.
Handling Bioreactors, reagent/media prep, sterile manufacturing,
upstream/downstream processing
Transfection, generate viral vectors, and efficient harvesting
Troubleshooting and optimizing processes stepwise.
Apply skills in cGMP and gene therapy, vector purification, process
scale-up, and sterile manufacturing
Bioprocess development of AAV vectors focusing, upstream
cultivation and harvest conditions to optimize yield
Managing suspension cultures, downstream purification recoveries,
and vector potency
Overall support of upstream process development for novel gene
therapy vector.
REQUIREMENTS:
Bachelor's (BS) or Master's (MS) in Molecular Biology, Virology,
Biology, Biochemistry or related
Minimum of a 4-8 yrs. + experience in the Biotech, Cell & Gene
Therapy (CGT) industry working with viral vectors
In depth knowledge of both upstream processing and or downstream
processing
Knowledge of protein purification, mammalian cell culture,
virology, viral validation, Preformulation
Formulation, ultracentrifugation, flocculation, scale-able
chromatography and tangential flow filtration (TFF).
Process Development (PD), manufacturing of viral vectors;
transfection, transcription, translation, AAV vector
in vivo genetic modification, Plasmid DNA Extraction
4-8 yrs. + experience handling bioreactors, media development, and
process development/ bioprocess refinement
4-8 yrs. + experience in protein and gene therapy, vector
purification, process scale-up, and sterile manufacturing
Upstream/Downstream and the equipment and tools used in that
process including; bioreactors, Vi-CELL, S.U.M.'s, sterile tubing
welders, filters, balances, microscopes, pipettes, CellSTACK's,
HYPERstack's Clipsters, DeltaV, BSC's, pH Meters, Osmometers,
Autoclaves etc.
Sterile manufacturing, cGMP, cleanroom, Upstream / Downstream
Process Development and GMP compliance.
UPSTREAM - 20L-200L bioreactors, cell expansion, transient
transfection, viral production and clarification
DOWNSTREAM -Recovery and purification of viral vectors and removal
of contaminants. Nuclease digestion, Purification, Sterile
Filtration and Fill
ANALYTICS - PAT, feed rates, pH control, Potency measurement,
Bioburden, Mycoplasma, Endotoxin
cGMP, sterility, fermentation, mammalian cell culture, bioprocess
optimization, purification, including clarification by filtration/
centrifugation; chromatography, ion exchange, sizing, multi-modal
and other resins
Tangential Flow Filtration (TFF) or Ultracentrifugation/
Diafiltration (UF/DF), Size Exclusion Chromatography.
PLEASE:
Share your updated resume using E-MAIL ADDRESS,
philip.cooke@yoh.com
CALL 860-405-7181 (anytime up until 7pm) to discuss
#ZipSPG
#LI-PC
Yoh, a Day & Zimmermann company, is an Equal Opportunity Employer.
All qualified applicants will receive consideration for employment
without regard to race, color, religion, sex, sexual orientation,
gender identity, national origin, disability, or status as a
protected veteran. Visit
https://www.yoh.com/applicants-with-disabilities to contact us if
you are an individual with a disability and require accommodation
in the application process.PandoLogic.Category: Science, Keywords:
Biochemist
Keywords: Yoh, A Day & Zimmermann Company, Medford , Upstream, Downstream Process Associate, Process Development, Vectors, Other , Medford, Massachusetts
Click
here to apply!
|
Didn't find what you're looking for? Search again!
Other Other JobsOrder Selector - Taunton, MA Description: Job DescriptionPosition Summary: An Order Selector is responsible for selecting product in a safe and efficient manner, which includes reading labels, placing product in the proper locations on pallets (more...) Company: Performance Food Group Location: Taunton Posted on: 01/18/2021 Physician / General Practice / New Hampshire / Locum tenens / Telemedi Description: Quivvy Tech is currently hiring telemedicine physicians Quivvy Tech is the fastest growing national telemedicine network in the U.S. We have Company: Quivvy Tech Location: Concord Posted on: 01/18/2021 Network System Engineer Description: Full-time position, 40 hours per week working Monday - Friday, 8 hour shifts. Please visit www.bidplymouth.org/careers to apply directly. Essential Duties and Responsibilities: -- Provides Senior technical (more...) Company: Beth Israel Deaconess Hospital Plymouth Location: Plymouth Posted on: 01/18/2021 Overnight Stocker Description: Job DescriptionPosition Purpose:Associates in Freight/Receiving positions ensure Company: The Home Depot Location: Plymouth Posted on: 01/18/2021 Travel CT Tech - $2,160 per week Description: MedPro Healthcare Allied Staffing is seeking a travel CT Tech for a travel job in Salem, Massachusetts.Job Description Requirements ul li Specialty: CT Tech li Discipline: Allied Health Professional (more...) Company: NurseFly Location: Salem Posted on: 01/18/2021 Wellpath: Residential Services Coordinator Description: Job DescriptionHere at Wellpath, everything that we do is about taking care of people our patients, our staff and our partners. We are committed to making a difference by providing hope and healing to (more...) Company: Wellpath Location: Plymouth Posted on: 01/18/2021 Wellpath: Residential Service Coordinator Description: Job DescriptionHere at Wellpath, everything that we do is about taking care of people our patients, our staff and our partners. We are committed to making a difference by providing hope and healing to (more...) Company: Wellpath Location: Plymouth Posted on: 01/18/2021 Physician / Family Practice / New Hampshire / Locum tenens / Telemedic Description: Quivvy Tech is currently hiring telemedicine physicians Quivvy Tech is the fastest growing national telemedicine network in the U.S. We have Company: Quivvy Tech Location: Concord Posted on: 01/18/2021 Loan Underwriter Description: Join a reputable mortgage company that offers a direct hire, six-figure income opportunity with full Company: Caliber Home Loans Location: Weymouth Posted on: 01/18/2021 Navy Chaplain Description: ABOUT The Navy Chaplain Corps boasts more than 800 Navy Chaplains from more than 100 different faith groups, including Christian, Jewish, Muslim, Buddhist and many others. Each Chaplain is also a Navy (more...) Company: Navy Location: Taunton Posted on: 01/18/2021 |