MedfordMARecruiter Since 2001
the smart solution for Medford jobs

Process Development Associate , Vectors

Company: Yoh, A Day & Zimmermann Company
Location: Medford
Posted on: February 20, 2021

Job Description:

Process Development Scientist ( Downstream, Bioprocess, Viral Vectors, AAV) 305174

RESPONSIBILITIES:


  • Bioprocess;- Downstream Process Development, process optimization for manufacturing viral vectors
  • Vector Engineering/ Viral Vectors. Support vector core Vector Engineering processes, Process Development
  • Support vector core vector engineering processes, process development, and process optimization
  • Process Development, process optimization, Bioprocess
  • Upstream/ Downstream Process Development, process optimization for manufacturing viral vectors
  • Bioprocess development of AAV vectors focusing, upstream cultivation and harvest conditions to optimize yield
  • Working with AAV viral particles, fermentation, plasmid recovery, purification and formulation, sterilization, and harvesting process steps
  • Apply research/ industry knowledge of Lentivirus (LV) and or Adeno Associated Virus (AAV)
  • Cell Line maintenance, Cell Line development, Media development; as cell are transfected with plasmids to generate viral vectors, which are efficiently harvested
  • Creating and maintaining stable cell lines
  • Troubleshooting and optimizing stepwise.
  • Plasmid Preparation, Buffer, Media Preparation, Aseptic Processing, growth & maintenance of cell lines.
  • Handling Bioreactors, reagent/media prep, sterile manufacturing, upstream/downstream processing
  • Transfection, generate viral vectors, and efficient harvesting
  • Troubleshooting and optimizing processes stepwise.
  • Apply skills in cGMP and gene therapy, vector purification, process scale-up, and sterile manufacturing
  • Bioprocess development of AAV vectors focusing, upstream cultivation and harvest conditions to optimize yield
  • Managing suspension cultures, downstream purification recoveries, and vector potency
  • Overall support of upstream process development for novel gene therapy vector.


    -

    -

    REQUIREMENTS:


    • Bachelor's (BS) or Master's (MS) in Molecular Biology, Virology, Biology, Biochemistry or related
    • Minimum of a 4-8 yrs. + experience in the Biotech, Cell & Gene Therapy (CGT) industry working with viral vectors
    • In depth knowledge of both upstream processing and or downstream processing
    • Knowledge of protein purification, mammalian cell culture, virology, viral validation, Preformulation
    • Formulation, ultracentrifugation, flocculation, scale-able chromatography and tangential flow filtration (TFF).
    • Process Development (PD), manufacturing of viral vectors; transfection, transcription, translation, AAV vector
    • in vivo genetic modification, Plasmid DNA Extraction
    • 4-8 yrs. + experience handling bioreactors, media development, and process development/ bioprocess refinement
    • 4-8 yrs. + experience in protein and gene therapy, vector purification, process scale-up, and sterile manufacturing
    • Upstream/Downstream and the equipment and tools used in that process including; bioreactors, Vi-CELL, S.U.M.'s, sterile tubing welders, filters, balances, microscopes, pipettes, CellSTACK's, HYPERstack's Clipsters, DeltaV, BSC's, pH Meters, Osmometers, Autoclaves etc.
    • Sterile manufacturing, cGMP, cleanroom, Upstream / Downstream Process Development and GMP compliance.
    • UPSTREAM - 20L-200L bioreactors, cell expansion, transient transfection, viral production and clarification
    • DOWNSTREAM -Recovery and purification of viral vectors and removal of contaminants. Nuclease digestion, Purification, Sterile Filtration and Fill
    • ANALYTICS - PAT, feed rates, pH control, Potency measurement, Bioburden, Mycoplasma, Endotoxin
    • cGMP, sterility, fermentation, mammalian cell culture, bioprocess optimization, purification, including clarification by filtration/ centrifugation; chromatography, ion exchange, sizing, multi-modal and other resins
    • Tangential Flow Filtration (TFF) or Ultracentrifugation/ Diafiltration (UF/DF), Size Exclusion Chromatography.


      -

      PLEASE:


      • Share your updated resume using E-MAIL ADDRESS,
      • CALL 860-405-7181 (anytime up until 7pm) to discuss


        -

        -

        -

        #ZipSPG

        #LI-PC

        Yoh, a Day & Zimmermann company, is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran. Visit https://www.yoh.com/applicants-with-disabilities to contact us if you are an individual with a disability and require accommodation in the application process.PandoLogic. Keywords: Biochemist, Location: Medford, MA - 02155

Keywords: Yoh, A Day & Zimmermann Company, Medford , Process Development Associate , Vectors, Other , Medford, Massachusetts

Click here to apply!

Didn't find what you're looking for? Search again!

I'm looking for
in category
within


Other Other Jobs


Military Police - Police Officer (31B)
Description: Have you ever dreamed of making an impact How about serving your community, State, and Nation As a Military Police Officer for the Army National Guard, you'll do all of these things by protecting, defending, (more...)
Company: Army National Guard
Location: Taunton
Posted on: 03/4/2021

Sales Associates
Description: Successful company experiencing rapid growth in the convenience store industry is currently looking to fill positions in your area Are you passionate about
Company: Nouria Energy
Location: Taunton
Posted on: 03/4/2021

15T UH-60 Utility Helicopter Repairer - Aviation
Description: The UH-60 Black Hawk helicopter is the best-in-class, multi-mission performer used for combat assault, search and rescue, medevac, and firefighting missions. Your responsibility as the UH-60 Utility (more...)
Company: Army National Guard
Location: Concord
Posted on: 03/4/2021


Weekday Caregiver
Description: There has never been a better time to consider joining our team here at Home Instead. Our CAREGivers have been providing front-line, exceptional care to our clients, and have made all the difference before (more...)
Company: Home Instead
Location: Concord
Posted on: 03/4/2021

Licensed Optician - ***Sign-On Bonus*** Job
Description: BJ's Wholesale Club was the first retailer to introduce the warehouse club concept in the northeastern United States. Today, we're a multibillion dollar operation with more than 200 clubs in 17 states (more...)
Company: BJ's Wholesale Club
Location: Weymouth
Posted on: 03/4/2021

92F Petroleum Supply Specialist - Supply and Logistics
Description: Fuel is one of the most vital components of the Army National Guard. It's what keeps Soldiers pushing onward, and as a Petroleum Supply Specialist, you'll make sure they'll never run out. In this position, (more...)
Company: Army National Guard
Location: Concord
Posted on: 03/4/2021

Warehouse Associate - 2nd Shift
Description: TriMark USA is one of the nation's largest providers of design and build services, equipment and supplies to the foodservice industry. TriMark offers operators an unrivaled level of customized service (more...)
Company: TriMark USA
Location: Taunton
Posted on: 03/4/2021

(Part Time) Meat Clerk Job
Description: BJ's Wholesale Club was the first retailer to introduce the warehouse club concept in the northeastern United States. Today, we're a multibillion dollar operation with more than 200 clubs in 17--states (more...)
Company: BJ's Wholesale Club
Location: Weymouth
Posted on: 03/4/2021

Cosmetic Merchandiser (part time)
Description: / Experienced Cosmetic Reset Merchandisers needed for temporary project with local retailer. positions are filling fast, ACT NOW br Atlantic Coast Merchandising is hiring temporary, part-time, project-based (more...)
Company: Atlantic Coast Merchandising
Location: Concord
Posted on: 03/4/2021

91B Light-Wheel Vehicle Mechanic - Mechanic and Maintenance
Description: As a Light-Wheel Vehicle Mechanic for the Army National Guard you'll ensure that anything that moves on wheels is ready to hit the road. In this role, you will primarily be responsible for supervising (more...)
Company: Army National Guard
Location: Concord
Posted on: 03/4/2021

Log In or Create An Account

Get the latest Massachusetts jobs by following @recnetMA on Twitter!

Medford RSS job feeds